FAQ's of Human EMMPRIN(Extracellular Matrix Metalloproteinase Inducer) ELISA Kit:
Q: How is the Human EMMPRIN ELISA Kit used in research?
A: The kit enables quantitative measurement of EMMPRIN (CD147/Basigin) in various biological samples, such as serum, plasma, tissue homogenates, and cell lysates, supporting studies in cell signaling, oncology, and biomarker discovery.
Q: What components are included in the kit upon delivery?
A: Each kit includes an antibody-coated 96-well microplate, standards, HRP conjugate, substrate, stop solution, sample diluent, wash buffer, positive and negative controls, and an instruction manual.
Q: Where is this kit applicable and what is its detection method?
A: This ELISA kit is designed for research settings and is applicable in biotechnology, pharmaceutical, and academic laboratories. It utilizes the Sandwich ELISA principle with colorimetric detection for accurate quantitation.
Q: What is the process for sample preparation and assay execution?
A: Samples, such as serum or plasma, are prepared according to the manual. You add 100 L per well, incubate, wash, add labeled reagents sequentially, and measure color development for analysis via the included standard curve.
Q: When should the kit be stored, and what are the storage recommendations?
A: Upon receipt, store the kit at 2-8C for use within 6 months or at -20C for longer shelf life. All reagents should remain sealed until use to maintain optimal performance.
Q: What are the benefits of using this Human EMMPRIN ELISA Kit?
A: Researchers benefit from high sensitivity, broad detection range, reproducible precision, reliable controls, and a complete package that ensures efficient, affordable, and accurate quantification.