FAQ's of Human HTRA4(High Temperature Requirement Factor A4) ELISA Kit:
Q: How is the Human HTRA4 ELISA Kit used in research applications?
A: The kit is utilized for the quantitative detection of HTRA4 protein levels in human samples, such as serum, plasma, tissue homogenates, and cell culture supernatant, following the sandwich ELISA method.
Q: What is the typical processing time for the HTRA4 ELISA Kit assay?
A: The complete assay can be performed within approximately 2-4 hours, with a protocol designed for research convenience and efficiency.
Q: Where should the kit be stored to maintain its effectiveness?
A: The kit should be stored at 2-8C, avoiding freeze/thaw cycles, to ensure reagent stability and optimal assay performance throughout its 6-12 months shelf life.
Q: What equipment is needed to measure results from this ELISA kit?
A: A standard microplate reader set to 450 nm is required to obtain colorimetric detection and quantify results as directed in the kit manual.
Q: How precise is the Human HTRA4 ELISA Kit, and what is its sensitivity?
A: The kit boasts intra- and inter-assay CVs of less than 10% and offers high sensitivity, usually less than 10 pg/mL, providing accurate and reproducible measurements.
Q: What are the benefits of using this ELISA kit over other detection methods?
A: The kit provides high accuracy, sensitivity, and specificity through antibody-antigen binding and covers a wide detection range, making it ideal for research applications focused on HTRA4 in human samples.