FAQ's of Human LEPR(Leptin Receptor) ELISA Kit:
Q: How is the Human LEPR (Leptin Receptor) ELISA Kit used in laboratory settings?
A: The kit is used to quantitatively detect human leptin receptor levels in serum, plasma, tissue homogenates, and cell culture supernatants using a sandwich ELISA protocol. Researchers add samples to the pre-coated plate, follow the provided steps, and measure absorbance at 450 nm.
Q: What are the key benefits of using the Human LEPR ELISA Kit?
A: This kit offers high sensitivity (<10 pg/ml), broad assay range (31.25-2000 pg/ml), and superior reproducibility (intra-assay CV <8%, inter-assay CV <10%). It is cost-effective, provides fast results in only 3.5 hours, and includes all necessary reagents for streamlined workflows.
Q: When should I aliquot and store the reagents after reconstitution?
A: After reconstitution, it is recommended to aliquot the reagents immediately and store them at -20C to maintain stability and ensure reliability for future experiments.
Q: What sample types are compatible with this ELISA Kit?
A: This ELISA kit is validated for serum, plasma, tissue homogenates, and cell culture supernatants, making it versatile for a wide range of research applications involving human samples.
Q: Where can I find detailed instructions for the assay process?
A: A comprehensive instruction manual is included with each kit, offering step-by-step guidance for sample preparation, reagent handling, assay execution, and result interpretation.
Q: What certifications and quality assurance does the kit have?
A: The Human LEPR ELISA Kit is research grade, confirmed by high purity (>98%) via both SDS-PAGE and HPLC, and is intended solely for research use, not diagnostic or therapeutic purposes.