LXB4(Lipoxin B4) ELISA Kit

This ELISA kit can be applied to the in vitro quantitative determination of  LXB4 concentrations in serum, plasma and other biological fluids.

This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with LXB4. During the reaction,  LXB4 in the sample or standard competes with a fixed amount of LXB4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to  LXB4. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of  LXB4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LXB4 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LXB4 were tested on 3 different plates, 20 replicates in each plate.

The unopened kit can be stored at 4 for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring.