FAQ's of Mouse CYR61(Cysteine Rich Protein, Angiogenic Inducer 61) ELISA Kit:
Q: How does the Mouse CYR61 ELISA Kit achieve high sensitivity and specificity?
A: The kit utilizes a sandwich ELISA format with HRP-conjugate and TMB substrate, providing high sensitivity (<10 pg/mL) and specificity towards natural and recombinant mouse CYR61 protein. The pre-coated 96-well plate and standardized calibration further enhance detection accuracy.
Q: What is the recommended process for sample preparation and usage of the kit?
A: Serum, plasma, cell culture supernatants, or tissue homogenates should be prepared according to the included instruction manual. Each well requires 50-100 L sample volume for optimal results, and the assay can be completed in approximately 2-3 hours with all essential reagents supplied in the kit.
Q: When should the kit be stored at 2-8C versus -20C?
A: While most components are stable at 2-8C, certain reagents may require storage at -20C for long-term stability. Refer to the kit-specific storage instructions provided to preserve component activity and maximize shelf life.
Q: Where is the Mouse CYR61 ELISA Kit manufactured and shipped from?
A: Manufacturing origin may vary depending on the supplier. The kit is shipped from established export hubs using secure shipment with ice packs (blue ice or dry ice as needed) to maintain reagent quality during transit.
Q: What are the key benefits of using the Mouse CYR61 ELISA Kit in research?
A: Researchers benefit from its high specificity and sensitivity, comprehensive reagent inclusion, user-friendly design, fast assay completion, and robust precision. It is ideal for studying angiogenesis, cell growth, and related biological processes in mouse models.