FAQ's of Mouse S100A8(S100 Calcium Binding Protein A8) ELISA Kit:
Q: How does the Mouse S100A8 ELISA Kit detect its target protein?
A: The kit employs a sandwich ELISA format using a specific capture and detection antibody pair to quantify S100A8, with a colorimetric readout measured at 450 nm.
Q: What types of samples can be analyzed with this kit?
A: This ELISA kit can be used with mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluids, enabling versatility in various research applications.
Q: Where should the Mouse S100A8 ELISA Kit be stored for maximum shelf life?
A: Store the kit at 2C to 8C as recommended, and avoid freeze-thaw cycles to preserve reagent integrity for up to 6-12 months.
Q: What is the typical detection range and sensitivity of this kit?
A: The typical detection range is 0.16-10 ng/mL, with a sensitivity down to 0.078 ng/mL, making it ideal for accurate quantification of low-abundance S100A8.
Q: How long does the assay process take from start to finish?
A: The complete assay process takes approximately 3.5 hours, providing a rapid and efficient workflow suitable for high-throughput research settings.
Q: When should standards and controls be included in the assay?
A: Standards and negative control must be included with every assay run to ensure accuracy, reproducibility, and reliable interpretation of results.