Product Description
Normal serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide, and freeze dried. Normal serum diluted to 5% (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific, conserved sequence and/or Fc receptor binding. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.
Physical State: Freeze-dried solid Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
Assured Sterility and SafetyOur Normal Horse Serum undergoes rigorous sterile filtration, mycoplasma testing, and viral screening, making it a safe and dependable supplement for sensitive biological applications. Free from added preservatives, it supports reproducible experimental results in immunology, microbiology, and cell culture research.
Consistent Composition and PurityWith a protein content ranging from 6.0 to 8.0 g/dL and a purity level of 99%, this serum ensures reliable and consistent supplementation for cell growth and metabolism. Its pale yellow to amber hue and characteristic smell guarantee its authenticity and high laboratory standards.
Flexible Packaging and Extended Shelf LifeAvailable in 100 ml, 500 ml, and 1 L bottles, Normal Horse Serum adapts to various scales of research. Properly stored at -20C, unopened serum maintains its integrity for up to five years, enhancing convenience for suppliers and laboratories alike.
FAQs of Normal Horse Serum:
Q: How should Normal Horse Serum be stored to ensure optimal preservation?
A: Normal Horse Serum must be stored at -20C in its original sealed bottle. This practice helps maintain its sterility, protein integrity, and effectiveness over its five-year shelf life.
Q: What is the recommended process for handling Normal Horse Serum in the laboratory?
A: To preserve sterility, always handle the horse serum in aseptic conditions inside a laminar flow hood. Use sterilized instruments and gloves to prevent contamination.
Q: When is Normal Horse Serum typically used in research?
A: It is commonly used as a supplement in cell culture media and in research pertaining to immunology and microbiology, providing essential growth factors and proteins required for cell maintenance.
Q: What are the primary benefits of using this serum without added preservatives?
A: The absence of preservatives minimizes the risk of interfering substances in cell culture, allowing for natural cell behavior. This makes the serum ideal for studies sensitive to chemical additives.
Q: Where can this Normal Horse Serum be sourced and what are its packaging options?
A: The product is distributed, imported, and traded within the United States, and is available in laboratory-grade bottles of 100 ml, 500 ml, and 1 L to accommodate different scales of research.
Q: What safety and quality tests are performed on the serum?
A: Every batch undergoes sterile filtration, screening for endotoxin levels under 10 EU/mL, confirming negative mycoplasma status, and testing for common horse viruses to ensure the highest level of safety and purity for research.