Product Description
Reactivity: Pig
Alternative Names: OL
Assay Type: Sandwich
Sensitivity: 0.56 ng/mL
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
High Sensitivity Sandwich ELISAThis kit utilizes a sandwich ELISA format with colorimetric detection, offering a sensitivity of less than 1 mU/ml and an assay range spanning from 1.56 to 100 mU/ml. Its specificity for oxidized LDL in pig samples ensures reliable and consistent results for your research applications.
Easy-to-Use Complete KitAll necessary reagents, including a pre-coated microplate, standards, detection antibody, and buffers, are included for convenience. The protocol is straightforward and well-supported by a comprehensive instruction manual, facilitating reproducibility and efficient workflow.
Reliable Results for ResearchWith intra-assay precision below 10% CV and inter-assay precision below 12% CV, this kit provides trustworthy and consistent quantitative results. It is ideal for use in research laboratories focused on cardiovascular or lipid-related studies in pigs.
FAQ's of Pig OxLDL(Oxidized Low Density Lipoprotein) ELISA Kit:
Q: How is the Pig OxLDL ELISA Kit utilized in research applications?
A: The Pig OxLDL ELISA Kit quantifies oxidized LDL in pig serum, plasma, or other biological fluids. Researchers can use this quantitative measurement to study lipid oxidation, a crucial factor in cardiovascular and metabolic research involving pigs.
Q: What is the detection method and sensitivity of this kit?
A: This kit employs a sandwich ELISA with colorimetric detection. It offers a high sensitivity of less than 1 mU/ml, allowing for accurate detection even at low oxidized LDL concentrations.
Q: When should samples or kit components be stored, and at what temperatures?
A: To maintain stability and performance, store all kit components at 2-8C upon receipt. It is important to avoid repeated freeze-thaw cycles to preserve reagent integrity.
Q: Where can the Pig OxLDL ELISA Kit be applied?
A: This kit is suitable for use in research laboratories conducting studies on lipid metabolism, oxidative stress, or cardiovascular health in Sus scrofa (pigs). It is not intended for in vivo or diagnostic use.
Q: What is the typical process for running this ELISA assay?
A: The process involves adding standards or samples to the pre-coated plate, applying detection antibody and enzyme conjugate, incubating, performing washes, and finally adding substrate and stop solution for colorimetric measurement. Detailed step-by-step protocols are provided in the instruction manual.
Q: How much sample is required per well in the assay?
A: Each well requires 50-100 L of sample, ensuring efficient use of your valuable biological materials while still providing reliable results.
Q: What are the benefits of using this ELISA kit for pig OxLDL?
A: Key benefits include high sensitivity, specific detection for pig samples, precise quantification, and user-friendly operation. The kit supports research into atherosclerosis and related conditions by enabling detailed analysis of oxidized LDL levels in porcine models.