Rat CASP8(Caspase 8) ELISA Kit

Name: Rat CASP8(Caspase 8) ELISA Kit
Brand: SR GROUP
Price: 18500 INR
Availability: InStock

Price 18500 INR/ Kit

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MOQ : 1 Piece

Rat CASP8(Caspase 8) ELISA Kit Specification

Type
ELISA Kit

Rat CASP8(Caspase 8) ELISA Kit Trade Information

Minimum Order Quantity
1 Piece

Supply Ability
500 Pieces Per Day

Delivery Time
1-7 Days

About Rat CASP8(Caspase 8) ELISA Kit

Rat CASP8(Caspase 8) ELISA Kit

Intended use

This ELISA kit applies to the in vitro quantitative determination of Rat CASP8 concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CASP8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CASP8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CASP8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Rat CASP8. You can calculate the concentration of Rat CASP8 in the samples by comparing the OD of the samples to the standard curve.

Assay type	Sandwich
Format	96T
Assay time	4.5h
Reactivity	Rat
Detection Method	Colormetric
Detection Range	0.16 10 ng/mL
Sensitivity	0.10 ng/mL
Sample Volume Required Per Well	100 L
Sample Type	Serum, plasma and other biological fluids

Specificity

This kit recognizes Rat CASP8 in samples. No significant cross-reactivity or interference between Rat CASP8 and analogues was observed.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(ng/mL)	O.D	Average	Corrected
10	2.437 2.461	2.449	2.389
5	1.653 1.667	1.66	1.6
2.5	0.969 0.957	0.963	0.903
1.25	0.437 0.475	0.456	0.396
0.63	0.263 0.263	0.263	0.203
0.32	0.177 0.163	0.17	0.11
0.16	0.109 0.123	0.116	0.056
0	0.054 0.066	0.06	--

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CASP8 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CASP8 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.48	1.38	4.76	0.44	1.46	4.79
Standard deviation	0.03	0.08	0.18	0.02	0.07	0.18
C V (%)	6.25	5.80	3.78	4.55	4.79	3.76

Recovery

The recovery of Rat CASP8 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum (n=5)	93-106	99
EDTA plasma (n=5)	92-103	97
Cell culture media (n=5)	87-98	92

Linearity

Samples were spiked with high concentrations of Rat CASP8 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	96-110	92-104	100-114
1:2	Average (%)	102	99	106
1:4	Range (%)	90-102	82-95	82-93
1:4	Average (%)	96	88	88
1:8	Range (%)	93-107	85-95	83-96
1:8	Average (%)	100	90	90
1:16	Range (%)	91-105	85-98	84-99
1:16	Average (%)	96	92	90

Kit components & Storage

An unopened kit can be stored at 4 for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	8 wells 12 strips	-20, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100 )	1 vial, 120 L
Concentrated HRP Conjugate (100 )	1 vial, 120 L	-20(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25 )	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	4(shading light)
Stop Solution	1 vial, 10 mL	4
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.

The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

Other supplies required

Microplate reader with 450 nm wavelength filter

High-precision transfer pipette, EP tubes and disposable pipette tips

Incubator capable of maintaining 37

Deionized or distilled water

Absorbent paper

Loading slot for Wash Buffer

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