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Rat GRO/CXCL1(Growth Regulated Oncogene Alpha) ELISA Kit

Rat GRO/CXCL1(Growth Regulated Oncogene Alpha) ELISA Kit
Rat GRO/CXCL1(Growth Regulated Oncogene Alpha) ELISA Kit
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Product Code : E-EL-R0003
Brand Name : Rat GRO/CXCL1
Price And Quantity
  • Minimum Order Quantity
  • 1
  • Price
  • 20000 INR
Product Specifications
  • Shelf Life
  • 12 Months
Product Description

Synonyms:NAP3, GRO1, GRO-A, MGSA, MGSA-A, SCYB1, FSP, CINC-1

Intended use

This ELISA kit applies to the in vitro quantitative determination of Rat GRO/CXCL1 concentrations in serum, plasma and other biological fluids.

Test principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GRO/CXCL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GRO/CXCL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GRO/CXCL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of Rat GRO/CXCL1. You can calculate the concentration of Rat GRO/CXCL1 in the samples by comparing the OD of the samples to the standard curve.

Assay type

Sandwich

Format

96T

Assay time

4.5h

Reactivity

Rat

Detection Method

Colormetric

Detection Range

15.631000 pg/mL

Sensitivity

9.38 pg/mL

Sample Volume Required Per Well

100 L

Sample Type

Serum, plasma and other biological fluids

Specificity

This kit recognizes Rat GRO/CXCL1 in samples. No significant cross-reactivity or interference between Rat GRO/CXCL1 and analogues was observed.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

Concentration(pg/mL)

O.D

Average

Corrected

1000

2.431
2.451

2.441

2.366

500

1.685
1.687

1.686

1.611

250

0.92
0.904

0.9119999999999999

0.837

125

0.495
0.533

0.514

0.439

62.5

0.273
0.247

0.26

0.185

31.25

0.186
0.168

0.177

0.102

15.63

0.121
0.133

0.127

0.052

0

0.07099999999999999
0.079

0.075

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Kit components & Storage

An unopened kit can be stored at 4 for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.


Item

Specifications

Storage

Micro ELISA Plate(Dismountable)

8 wells 12 strips

-20, 6 months

Reference Standard

2 vials

Concentrated Biotinylated Detection Ab (100)

1 vial, 120 L

Concentrated HRP Conjugate (100)

1 vial, 120 L

-20(shading light), 6 months

Reference Standard & Sample Diluent

1 vial, 20 mL

4, 6 months

Biotinylated Detection Ab Diluent

1 vial, 14 mL

HRP Conjugate Diluent

1 vial, 14 mL

Concentrated Wash Buffer (25)

1 vial, 30 mL

Substrate Reagent

1 vial, 10 mL

4(shading light)

Stop Solution

1 vial, 10 mL

4

Plate Sealer

5 pieces


Product Description

1 copy

Certificate of Analysis





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