This ELISA kit applies to the in vitro quantitative determination of Rat IL-1Î² concentrations in serum, plasma and other biological fluids.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1Î². Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-1Î² and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-1Î², biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm Â± 2 nm. The OD value is proportional to the concentration of Rat IL-1Î². You can calculate the concentration of Rat IL-1Î² in the samples by comparing the OD of the samples to the standard curve.
|Detection Range||31.25â2000 pg/mL|
|Sample Volume Required Per Well||100 Î¼L|
|Sample Type||Serum, plasma and other biological fluids|
This kit recognizes Rat IL-1Î² in samples. No significant cross-reactivity or interference between Rat IL-1Î² and analogues was observed.
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
An unopened kit can be stored at 4â for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.
|Micro ELISA Plate(Dismountable)||8 wells Ã12 strips||-20â, 6 months|
|Reference Standard||2 vials|
|Concentrated Biotinylated Detection Ab (100Ã)||1 vial, 120 Î¼L|
|Concentrated HRP Conjugate (100Ã)||1 vial, 120 Î¼L||-20â(shading light), 6 months|
|Reference Standard & Sample Diluent||1 vial, 20 mL||4â, 6 months|
|Biotinylated Detection Ab Diluent||1 vial, 14 mL|
|HRP Conjugate Diluent||1 vial, 14 mL|
|Concentrated Wash Buffer (25Ã)||1 vial, 30 mL|
|Substrate Reagent||1 vial, 10 mL||4â(shading light)|
|Stop Solution||1 vial, 10 mL||4â|
|Plate Sealer||5 pieces|
|Product Description||1 copy|
|Certificate of Analysis||1 copy|