FAQ's of Rat INS(Insulin) ELISA Kit:
Q: How is the Rat INS (Insulin) ELISA Kit typically used in research?
A: The kit is employed for quantitative measurement of insulin in rat serum, plasma, tissue homogenates, and cell culture supernatants. It supports studies in metabolic research, diabetes models, and biomarker validation by providing precise insulin quantification in controlled laboratory settings.
Q: What sample volumes and types are compatible with this kit?
A: The kit requires 50-100 L per well and accommodates serum, plasma, tissue homogenates, cell lysates, and cell culture supernatants derived from rat samples, ensuring broad applicability for diverse research needs.
Q: When can I expect results after starting the assay?
A: Depending on the protocol selected, results are available within 2 hours for the short protocol or 2-5 hours using the standard procedure-ideal for researchers needing timely turnaround.
Q: Where should the kit and its components be stored upon arrival?
A: Store all kit components at 2-8C. It is essential to avoid repeated freeze-thaw cycles to preserve reagent stability over its 6-12 months shelf life.
Q: What detection method does this ELISA kit use?
A: This kit utilizes HRP-based colorimetric detection (TMB substrate), generating a measurable signal at 450 nm that correlates with the concentration of insulin present in the sample.
Q: How does the kit ensure precision in results?
A: The Rat INS ELISA Kit achieves high precision with both intra- and inter-assay coefficient of variation (CV) under 10%, ensuring reliable and repeatable measurements every time.