FAQ's of Rat RANTES(Regulated On Activation In Normal T-Cell Expressed And Secreted) ELISA Kit:
Q: How does the Rat RANTES ELISA Kit detect CCL5 levels?
A: The kit utilizes a sandwich ELISA format, where the target antigen binds to a pre-coated antibody. Detection is achieved through HRP-conjugated reagents and colorimetric substrates, allowing precise quantification.
Q: What sample types are compatible with this ELISA kit?
A: This kit is compatible with serum, plasma, tissue homogenates, cell lysates, and cell culture supernatants derived from rat specimens.
Q: When should I use the positive controls included in the kit?
A: Positive controls should be run with each assay to validate accuracy, ensuring the reliability of test results for both new and routine experiments.
Q: Where should I store the Rat RANTES ELISA Kit for optimal stability?
A: Store the boxed kit at 2-8C away from light to preserve reagent integrity and extend its shelf life to 6-12 months.
Q: What are the main benefits of purchasing this ELISA kit for research?
A: Researchers benefit from high sensitivity (<5 pg/mL), precision, a complete set of reagents, and user-friendly instructions, making it ideal for robust quantification of CCL5 in diverse rat study models.
Q: How long does the complete assay take to perform?
A: The total incubation and reaction time required is typically between 2-4 hours, depending on the detailed protocol followed for sample processing.