Zika Real Time RT-PCR Kit

Product Details:

Storage -20C
Usage In vitro diagnostic use only
Shelf Life 12 months
Appearance Kit Form (contains multiple reagents, vials)
Application Detection of Zika virus RNA in clinical samples
Other Names Zika Virus Detection Kit
HS Code 3822
Click to View more
Share Your Product:

Request to Call Back Send Inquiry

Zika Real Time RT-PCR Kit Product Specifications

HS Code 3822
Grade Molecular Diagnostics Grade
Shelf Life 12 months
Usage In vitro diagnostic use only
Storage -20C
Type PCR Kit
Other Names Zika Virus Detection Kit
Application Detection of Zika virus RNA in clinical samples
Appearance Kit Form (contains multiple reagents, vials)
Classification Diagnostic Reagent
Poisonous No
Physical Form Liquid and Lyophilized components
Chemical Name Zika Real Time RT-PCR Kit

Product Description

Product Name		Zika, PCR Kit
Also Known As		Zika Real Time RT-PCR Kit
Research Use Only		For Research Use Only. Not for use in diagnostic procedures.

		PRICE -76,950 FOR 25 TEST PRODUCT DELIVERY - 10-12 DAYS



Samples		Serum or plasma by using real time PCR systems.

Preparation and Storage		All reagents should be stored at -20 degree C. Storage at +4 degree C is not recommended. Please refer to the pdf manual for more specific storage instructions.
ISO Certification		Manufactured in an ISO 13485 Certified Laboratory.
Other Notes		Small volumes of Zika pcr kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
TOP

Product Description specifically for Zika pcr kit		Intended Uses: Zika Virus (ZIKV) Real Time RT-PCR Kit is used for the detection of Zika virus in serum or plasma by using real time PCR systems. Principle of the Assay\|\|The principle of the real-time detection is based on the fluorogenic 5'nuclease assay. During the PCR reaction, the DNA polymerase cleaves the probe at the 5' end and separates the reporter dye from the quencher dye only when the probe hybridizes to the target DNA. This cleavage results in the fluorescent signal generated by the cleaved reporter dye, which is monitored real-time by the PCR detection system. The PCR cycle at which an increase in the fluorescence signal is detected initially is proportional to the amount of the specific PCR product. Monitoring the fluorescence intensities in real-time allows the detection of the accumulating product without having to re-open the reaction tube after the amplification

pack size-

25 Tests/Kit (Instr. I, II Recommended) / $570 +1 FREE 8GB USB
25 Tests/Kit (Instr. III, IV Recommended) / $570 +1 FREE 8GB USB

4. Kit Contents- for 25 rxn

Ref. Type of reagent Presentation 25rxns

ZIKV Super Mix RT-PCR-1 vial, 350ul

RT-PCR Enzyme Mix- 1 vial, 28ul

Molecular Grade Water-1 vial, 400l

Internal Control (IC)-1 vial, 30l

ZIKV Positive Control(1107 copies/ml) -1 vial, 30l

Analysis sensitivity:

5103 copies/ml LOQ11041108 copies/ml Note: Analysis sensitivity depends on the sample volume, elution volume, nucleic acid extraction methods and other factors .If you use the RNA extraction kits recommended, the analysis sensitivity is the same as it declares. However, when the sample volume is dozens or even hundreds of times greater than elution volume by some concentrating method, it can be much higher

Accurate Zika Virus Detection

This kit utilizes real-time reverse transcriptase polymerase chain reaction (RT-PCR) technology, ensuring precise detection of Zika virus RNA. Its specialized primers and probes provide high specificity, minimizing the risk of cross-reactivity with other viruses. The high sensitivity allows for detection of as few as 100 copies per reaction, which is crucial for early diagnosis and disease management.

Comprehensive Kit Components

The Zika Real Time RT-PCR Kit includes all necessary reagents for successful amplification and detection. It comes with a RT-PCR master mix, enzyme mix, primers, probes, and both positive and negative controls. Each component is quality-tested, ensuring reliable performance in clinical diagnostics. The kit also contains a detailed instruction manual for streamlined workflow.

User-Friendly and Reliable

Designed for ease of use, the kit is compatible with widely used real-time PCR platforms. Detailed instructions facilitate a straightforward process, making it suitable for experienced lab professionals and new users alike. The reagents are provided in liquid and lyophilized forms, optimizing stability and convenience during storage and handling.

FAQs of Zika Real Time RT-PCR Kit:

Q: How does the Zika Real Time RT-PCR Kit detect Zika virus?

A: The kit uses real-time reverse transcriptase polymerase chain reaction (RT-PCR) technology to amplify and specifically detect Zika virus RNA in clinically relevant samples such as serum, plasma, urine, and others.

Q: What are the storage requirements for the kit components?

A: All reagents must be stored at -20C to maintain their stability and performance. The kit has a shelf life of 12 months when stored under recommended conditions.

Q: When can I expect the results after starting the RT-PCR assay?

A: From sample to result, the amplification process takes approximately 1.5 hours, making it possible to obtain results within the same working day.

Q: Where can the kit be used, and who is the intended user?

A: The kit is intended for use in diagnostic laboratories and research settings by trained professionals experienced in real-time PCR techniques. It is distributed, imported, and supplied across India.

Q: What are the benefits of using this RT-PCR kit for Zika virus detection?

A: This kit offers high sensitivity (detects 100 copies/reaction) and specificity for Zika virus RNA, ensuring reliable results. The inclusion of all necessary reagents, controls, and an instruction manual simplifies the testing process and increases diagnostic confidence.

Q: How should I transport and handle the kit upon arrival?

A: The kit is shipped on ice packs to preserve reagent quality. Upon arrival, promptly transfer the kit to a -20C freezer for proper storage.

Q: Can this kit be used for any type of sample?

A: The kit has been validated for use with serum, plasma, urine, and other recommended clinical specimens. Always refer to the instruction manual for approved sample types and preparation guidelines.