FAQ's of Mouse VE-Cadherin(Vascular Endothelial Cadherin) ELISA Kit:
Q: How should the Mouse VE-Cadherin ELISA Kit be stored?
A: The kit components must be kept refrigerated at 2-8C. Avoid freezing components unless specifically indicated in the kit manual to maintain reagent integrity.
Q: What is the primary application of this ELISA kit?
A: This kit is designed for the quantitative measurement of VE-Cadherin in mouse serum, plasma, tissue homogenates, cell lysates, and cell culture supernatants using a sandwich ELISA format.
Q: When is the kit most commonly used in research?
A: Researchers commonly use this kit when studying vascular endothelial function, disease mechanisms, or cell adhesion processes in mouse models.
Q: Where can I find the Certificate of Analysis for this kit?
A: A Certificate of Analysis is available on request from your distributor, importer, supplier, or trader. The lot number is specified on the kit label for your reference.
Q: What is the process for performing the assay?
A: Add standards, samples, and controls into pre-coated wells, then sequentially add detection antibody, enzyme conjugate, and substrate. Color development is read at 450 nm using a microplate reader.
Q: What are the main benefits of this ELISA kit?
A: The kit offers high sensitivity (<10 pg/mL), excellent precision (CV <12%), standardized calibration to recombinant VE-Cadherin, and compatibility with standard laboratory instrumentation, ensuring robust data and reproducible results.