Rabbit Anti-Dog IgG (H+L)

Rabbit Anti-Dog IgG (H+L)

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Rabbit Anti-Dog IgG (H+L)

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MOQ : 1 Pack

Rabbit Anti-Dog IgG (H+L) Specification

Ph Level
7.27.4 (in PBS Buffer)

Application
ELISA, Western blot, Immunohistochemistry, Immunofluorescence

Appearance
Liquid

Usage
Detection of Dog IgG

Molecular Weight
~150 kDa

Poisonous
No

Shelf Life
12 Months

Grade
Research Grade

Smell
Odorless

Other Names
Polyclonal Rabbit Anti-Dog Immunoglobulin G (Heavy and Light Chains)

Physical Form
Solution

HS Code
3822

Storage
Store at -20C to -80C

Classification
Immunoglobulin

Chemical Name
Rabbit Anti-Dog IgG (H+L)

Type
Antibody

Purity(%)
> 95% (SDS-PAGE)

Color
Colorless to pale yellow

Formulation
Supplied as a liquid solution

Concentration
1 mg/mL (typical)

Buffer
Phosphate Buffered Saline (PBS), pH 7.2, with 0.01% Thimerosal as preservative

Target Species
Dog

Conjugation
Unconjugated (unless otherwise specified)

Host
Rabbit

Reactivity
Broad reactivity to Dog IgG heavy and light chains

Preservative
0.01% Thimerosal

Endotoxin Level
<2 EU/mg

Filter Sterilized
Yes

Isotype
Polyclonal IgG (H+L)

Affinity
Affinity purified

Immunogen
Purified Dog IgG

About Rabbit Anti-Dog IgG (H+L)

Product Specifications

Product: Rabbit Anti-Dog IgG (H+L)

Code Number: 304-001-003

Lot Number: 97523

Physical State: Freeze-dried solid

Size: 2.0 ml

Protein Concentration: 25.0 mg/ml

Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6

Preservative: 0.05% Sodium Azide

Reconstitution and Storage: Store freeze-dried powder at 2-8C. When ready to use, rehydrate with 2.0 ml dH2O and centrifuge if

not clear. Product is stable for about 6 weeks at 2-8C as an undiluted liquid. Prepare working dilution fresh each day. For

extended storage after rehydration, aliquot and freeze undiluted product at -20C or below. Avoid repeated freezing and thawing.

Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the

intended use.

Purity: The antibody was delipidated and ammonium sulfate fractionated from whole antiserum.

Antibody Specificity: Based on immunoelectrophoresis, the antibody reacts with whole molecule dog IgG. It also reacts with the

light chains of other dog immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody

may cross-react with immunoglobulins from other species.

Country of Origin: USA

Note: For in vitro research use only, not for diagnostic or therapeutic use. This product is not a medical device.

Exceptional Affinity and Purity

This product undergoes affinity purification to ensure selective binding to dog IgG (H+L) with minimized cross-reactivity. Its purity exceeds 95% as verified by SDS-PAGE, ensuring high performance and specificity in immunological studies.

Versatile Application Spectrum

Rabbit Anti-Dog IgG (H+L) is commonly used in ELISA, Western blot, immunofluorescence, and immunohistochemistry assays. Its broad reactivity to both heavy and light chains of dog IgG makes it highly suitable for various detection and analytical protocols in research environments.

Reliable and Safe Formulation

Supplied as a colorless to pale yellow liquid in PBS at pH 7.2, this antibody contains 0.01% Thimerosal to maintain stability without risks associated with hazardous substances. It is odorless, filter sterilized, and non-poisonous, ensuring safety during laboratory handling.

FAQs of Rabbit Anti-Dog IgG (H+L):

Q: How should I store Rabbit Anti-Dog IgG (H+L) to maintain its stability?

A: For optimal stability and performance, Rabbit Anti-Dog IgG (H+L) should be stored at temperatures between -20C and -80C. Avoid repeated freeze-thaw cycles to preserve its integrity throughout its 12-month shelf life.

Q: What applications can Rabbit Anti-Dog IgG (H+L) be used for?

A: This antibody is suitable for a range of immunological applications, including ELISA, Western blot, immunohistochemistry, and immunofluorescence for the detection of dog IgG.

Q: When is it recommended to use an unconjugated antibody such as this one?

A: An unconjugated antibody is preferred for flexible downstream detection, allowing for choice in secondary detection systems or specialized assay requirements not restricted to a particular label or enzyme.

Q: Where does the broad reactivity of this antibody provide benefits in research?

A: The broad reactivity to both heavy and light chains of dog IgG ensures comprehensive detection across varied experimental protocols, supporting studies involving diverse samples or detection methods.

Q: What is the process behind the affinity purification of this antibody?

A: Affinity purification involves selectively isolating Rabbit Anti-Dog IgG (H+L) antibodies that specifically bind to dog IgG, enhancing specificity and reducing background in assays.

Q: How is the antibodys purity verified, and why is it important?

A: Purity is assessed via SDS-PAGE and confirmed to exceed 95%. High purity minimizes background noise and non-specific binding, improving experimental accuracy.

Q: What are the benefits of the antibodys filter sterilization and low endotoxin level?

A: Filter sterilization ensures the antibody solution is free of microbial contaminants, and low endotoxin levels (<2 EU/mg) make it suitable for sensitive biological assays without eliciting unwanted immune responses.

Rabbit Anti-Dog IgG (H+L)

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