SEM(Nitrofurazone Metabolite) ELISA Kit

About SEM(Nitrofurazone Metabolite) ELISA Kit

Test principle

This kit uses Indirect-Competitive-ELISA as the method. It can detect SEM in samples, such as honey, tissue, milk, fodder, etc. This kit is composed of MicroELISA Plate, HRP conjugate, antibody, standard and other supplementary reagents. The micro-plate provided in this kit has been pre-coated with SEM. During the reaction, SEM in the samples or standard competes with SEM on the solid phase supporter for sites of SEM antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of SEM. The concentration of SEM in the samples can be calculated by comparing the OD of the samples to the standard curve.

Technical indicator

Sensitivity: 0.05 ppb (ng/mL)

Reaction mode: 25, 45 min~ 15 min

Detection limit: Tissue, liver---0.1 ppb, Honey/milk/casing---0.1 ppb,

                             Milk powder/egg powder/fodder---0.1 ppb, Fish and shrimp---0.15 ppb

Cross-reactivity: Nitrofurazone metabolite---100%, Furazolidone metabolite---0.1%,

                                Nitrofurantoin metabolite---0.1%, Furaltadone metabolite---0.1%

Sample recovery rate: Tissue/liver---90%15%,Honey/milk/casing---80%15%; 

                                          Milk powder/egg powder/fodder--85%25%

Kits components

Other supplies required

Instruments: Micro-plate reader, Printer, Homogenizer, Nitrogen Evaporators, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).

High-precision transferpettor: Single channel (20-200 L, 100-1000 L), Multichannel (300 L).

Reagents: Ethyl acetate, N-hexane, Sodium hydroxide, Concentrated HCl, K2HPO43H2O, K2Fe (CN) 5(NO) 2H2O, ZnSO47H2O.