Goat Anti-Rat IgG, Fc fragment specific

9350 INR

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Product Details:

Storage Store at -20C for long-term, 2-8C for short-term
Shelf Life 12 months (lyophilized), 6-12 months (liquid)
HS Code 38220090
Ph Level 7.2-7.4
Smell Odorless
Physical Form Aqueous solution or powder
Application ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
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Goat Anti-Rat IgG, Fc fragment specific Price And Quantity

Minimum Order Quantity 1 Bottle
Price Range 9350.00 - 17500.00 INR
Price 9350 INR

Goat Anti-Rat IgG, Fc fragment specific Product Specifications

Color Colorless to pale yellow
Storage Store at -20C for long-term, 2-8C for short-term
Shelf Life 12 months (lyophilized), 6-12 months (liquid)
HS Code 38220090
Ph Level 7.2-7.4
Smell Odorless
Classification Immunoglobulin G antibody
Physical Form Aqueous solution or powder
Application ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Type Secondary Antibody
Poisonous Non-poisonous
Chemical Name Goat Anti-Rat IgG, Fc fragment specific
Grade Research Grade
Purity(%) >95% (by SDS-PAGE)
Appearance Liquid or lyophilized powder
Molecular Weight ~150 kDa
Usage Detection and quantification of Rat IgG Fc domain
Other Names Goat Anti-Rat IgG, Fc

Product Description

Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide and freeze-dried. Antisera against whole serums are obtained by immunizing host animals with whole serum. Antisera against whole IgG molecules [i.e. Anti-IgG (H+L)] are recommended for bridging PAP to primary antibodies.

Based on immunoelectrophoresis, the antibody reacts with the Fc portion of rat IgG heavy chain but not with the Fab portion of rat immunoglobulins. No antibody was detected against rat IgM or against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Physical State: Freeze-dried solid
Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.

Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

Purity: The antibody was delipidated and ammonium sulfate fractionated from whole antiserum.
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Preservative: 0.05% Sodium Azide

Accurate and Specific Detection

This secondary antibody targets the Fc region of rat IgG with high specificity, minimizing background and cross-reactivity with immunoglobulins of other species. It is ideal for research applications requiring precise quantification and detection of rat IgG, such as in ELISA, Western Blot, and immunohistochemistry.

Optimal Formulation and Purity

Supplied in sterile PBS solution (pH 7.2) and filtered for purity, the antibody is available as a liquid or lyophilized powder. With a purity exceeding 95% by SDS-PAGE and endotoxin levels under 0.1 EU/g, it ensures consistent and reliable results in sensitive applications.

Flexible Usage Across Applications

Tested for ELISA, Western Blot, IHC, and IP, this antibody adapts to multiple immunoassay platforms. Recommended dilution is 1:1,0001:10,000 for ELISA, though protocols may require optimization. The products stability is maintained with sodium azide preservative, making it suitable for both routine and specialized research needs.

FAQs of Goat Anti-Rat IgG, Fc fragment specific:

Q: How should Goat Anti-Rat IgG, Fc fragment specific be stored for optimal stability?

A: For long-term storage, keep the antibody at -20C. For use within a few weeks, store at 28C. Avoid repeated freeze-thaw cycles to maintain antibody integrity and potency.

Q: What applications is this antibody validated for?

A: This reagent is tested and validated for ELISA, Western Blot, Immunohistochemistry (IHC), Immunoprecipitation, and Immunofluorescence, making it a versatile tool for detection and quantification of rat IgG Fc regions.

Q: When should I use the liquid versus the lyophilized form of this antibody?

A: The liquid form is ready to use for immediate applications, while the lyophilized powder is suited for long-term storage and shipments. Both forms provide equivalent performance after proper reconstitution.

Q: What is the recommended dilution for ELISA, and does it vary by application?

A: For ELISA, a starting dilution of 1:1,0001:10,000 is recommended. The optimal dilution may require adjustment based on specific protocols and assay conditions in Western Blot, IHC, or IP.

Q: Where can I obtain a Certificate of Analysis for this product?

A: A Certificate of Analysis, detailing product quality and performance specifications, is available upon request from your distributor, supplier, or importer.

Q: Why choose an Fc specific secondary antibody like this?

A: Fc specificity ensures minimal cross-reactivity, allowing precise detection of the desired IgG subclass and reducing background signal, thereby enhancing assay accuracy and reproducibility.

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