Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide and freeze-dried. Antisera against whole serums are obtained by immunizing host animals with whole serum. Antisera against whole IgG molecules [i.e. Anti-IgG (H+L)] are recommended for bridging PAP to primary antibodies.
Based on immunoelectrophoresis, the antibody reacts with the Fc portion of rat IgG heavy chain but not with the Fab portion of rat immunoglobulins. No antibody was detected against rat IgM or against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.Physical State: Freeze-dried solid
Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.Purity: The antibody was delipidated and ammonium sulfate fractionated from whole antiserum.