Goat Anti-Rat IgG (H+L)

Goat Anti-Rat IgG (H+L)

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Goat Anti-Rat IgG (H+L)

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Goat Anti-Rat IgG (H+L) Specification

Structural Formula
Protein Structure (not available in chemical formula)

Ph Level
7.2-7.4 (Phosphate Buffered Saline)

Other Names
Goat Anti-Rat IgG (Heavy and Light chains), Secondary Antibody

Appearance
Liquid Solution or Lyophilized Powder

Usage
Detection of Rat IgG in various immunoassays

Molecular Weight
Approx. 150 kDa

HS Code
38220000

Shelf Life
12 months from date of manufacture

Storage
2-8°C (Short-term); -20°C (Long-term)

Smell
Odorless

Poisonous
Non-Poisonous

Application
Immunohistochemistry, ELISA, Western Blot, Immunofluorescence

Grade
Research Grade

Physical Form
Liquid or Lyophilized

Classification
Antibody

Chemical Name
Goat Anti-Rat Immunoglobulin G (IgG) (H+L)

Type
Polyclonal Secondary Antibody

Purity(%)
>95% (by SDS-PAGE)

Color
Colorless or Slightly Yellow

Cross Reactivity
Minimal cross-reactivity with human, mouse, rabbit, and bovine IgG

Clonality
Polyclonal

Buffer
PBS (Phosphate Buffered Saline), may contain preservatives

Preservative
0.02% Sodium Azide

Endotoxin Level
<1.0 EU/mg

Recommended Dilution
1:1000-1:5000 (ELISA), 1:500-1:2000 (WB/IHC)

Target Species
Rat

Safety Information
Not hazardous under normal laboratory conditions

Immunogen
Purified rat IgG (H+L)

Packaging Size
Available in 0.5 mL, 1 mL, and bulk quantities

Host Species
Goat

Conjugation
Unconjugated (other conjugates available on request)

Certificate of Analysis
Available on request

About Goat Anti-Rat IgG (H+L)

Polyclonal antisera from immunized hosts are lipid extracted to improve clarity, salt fractionated, dialyzed against phosphate buffered saline containing sodium azide and freeze-dried. Antisera against whole serums are obtained by immunizing host animals with whole serum. Antisera against whole IgG molecules [i.e. Anti-IgG (H+L)] are recommended for bridging PAP to primary antibodies.

Based on immunoelectrophoresis, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

Physical State: Freeze-dried solid
Storage: Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing.

Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

Reliable Detection Across Immunoassays

Goat Anti-Rat IgG (H+L) provides robust performance in ELISA, Western blot, immunohistochemistry, and immunofluorescence. Engineered for high specificity and minimal cross-reactivity, this secondary antibody ensures accurate detection of rat IgG in complex samples, enhancing the reliability of your results in diverse research workflows.

High Purity and Flexible Formats

With over 95% purity (SDS-PAGE), the antibody is available as a colorless or slightly yellow liquid solution or as a lyophilized powder. It can be ordered in convenient 0.5 mL and 1 mL packaging, as well as bulk quantities to fit your laboratorys scale and application needs.

Safe, Stable, and Easy to Store

This research grade antibody is odorless, non-poisonous, and safe for typical laboratory handling. It is stable for up to 12 months when stored at 2-8C for short term or -20C for long term. A certificate of analysis is available upon request, ensuring product quality and compliance.

FAQs of Goat Anti-Rat IgG (H+L):

Q: How should Goat Anti-Rat IgG (H+L) be stored for maximum stability?

A: For optimal stability, store the antibody at 2-8C for short-term use. For long-term storage, it is best kept at -20C. This ensures the antibody maintains its activity and minimizes degradation.

Q: What applications is this secondary antibody suitable for?

A: Goat Anti-Rat IgG (H+L) is suitable for ELISA, Western blot, immunohistochemistry, and immunofluorescence. It facilitates sensitive detection of rat IgG in a variety of immunoassays.

Q: When should I use this secondary antibody over others?

A: This antibody is ideal when high specificity to rat IgG is required, especially in assays with potential exposure to other species immunoglobulins. Its minimal cross-reactivity reduces background interference from human, mouse, rabbit, or bovine IgGs.

Q: Where can I purchase Goat Anti-Rat IgG (H+L), and in which sizes is it available?

A: This product is available through a range of distributors, importers, suppliers, and traders in the United States. It comes in packaging sizes of 0.5 mL, 1 mL, and bulk quantities to accommodate different experimental needs.

Q: What are the recommended working dilutions for this antibody?

A: For ELISA, dilutions of 1:1000 to 1:5000 are recommended. For Western blot and immunohistochemistry, use between 1:500 and 1:2000. Always optimize dilutions based on your specific protocol and detection system.

Q: How does this antibody ensure minimal cross-reactivity in experiments?

A: The antibody is specifically tested to have minimal cross-reactivity with human, mouse, rabbit, and bovine IgG, making it highly selective for rat IgG and advantageous in experiments involving multiple species.

Q: What documentation is provided with the product to support research requirements?

A: A certificate of analysis detailing the quality and specifications of the antibody is available upon request, ensuring compliance and traceability in research documentation.

Goat Anti-Rat IgG (H+L)

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